
Introduction:
Cellulase is obtained by submerged fermentation from Trichoderma Reesei with advanced post-processing techniques. It is composed of multiple ingredients and can degrade cellulose in fibers to create simple sugars efficiently. Cellulase is widely used in food, juice, phytoextraction, ethanol, textile and feed products, etc.
Function
Cellulase system consists of three major components: Endoglucanases (endo-1,4-β-D-glucanases; C1; EG, EC 3.2.1.4), cellobiohydrolases (exo-1,4-β-D-glucanases; CX; CBH, EC 3.2.1.91), and β-glucosidases (1,4-β-D-glucosidase; βG; BGL, EC 3.2.1.21). EG acts on insoluble cellulose surface, breaks internal bonds to disrupt the crystalline structure of cellulose and expose individual cellulose polysaccharide chains, and makes cellulose chains hydration easily. CBH cleaves 2-4 units from the ends of the exposed chains produced by EG, resulting in the tetrasaccharides or disaccharide such as cellobiose. There are two main types of CBH- one type working progressively from the reducing end, and the other type working progressively from the non-reducing end of cellulose. βG hydrolyses the CBH product into individual monosaccharides. Through the synergistic action of above enzyme system, cellulose can be efficiently hydrolyzed to glucose.
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